Escherichia coli RecQ Is a Rapid, Efficient, and Monomeric Helicase
نویسندگان
چکیده
منابع مشابه
The Escherichia coli RecQ helicase functions as a monomer.
The RecQ helicases belong to an important family of highly conserved DNA helicases that play a key role in chromosomal maintenance, and their defects have been shown to lead to several disorders and cancer in humans. In this work, the conformational and functional properties of the Escherichia coli RecQ helicase have been determined using a wide array of biochemical and biophysical techniques. ...
متن کاملRecQ DNA helicase is a suppressor of illegitimate recombination in Escherichia coli.
Bloom syndrome and Werner syndrome are genetic disorders in which an increased rate of chromosomal abnormality is observed. The genes responsible for these diseases, BLM and WRN, have been cloned and identified as homologs of the Escherichia coli recQ genes. We studied the effect of recQ mutations on illegitimate recombination, which is an aberrant biological event related to the chromosomal ab...
متن کاملBiochemical characterization of the DNA helicase activity of the escherichia coli RecQ helicase.
We demonstrate that RecQ helicase from Escherichia coli is a catalytic helicase whose activity depends on the concentration of ATP, free magnesium ion, and single-stranded DNA-binding (SSB) protein. Helicase activity is cooperative in ATP concentration, with an apparent S(0.5) value for ATP of 200 microm and a Hill coefficient of 3.3 +/- 0.3. Therefore, RecQ helicase utilizes multiple, interact...
متن کاملCloning and characterization of RECQL, a potential human homologue of the Escherichia coli DNA helicase RecQ.
A potential human DNA helicase, RECQL, was partially purified from HeLa cells, and a cDNA encoding this protein was subsequently cloned from a HeLa library. The RECQL cDNA contains a protein coding region of 1977 base pairs, and encodes a 659-amino-acid polypeptide with a predicted M(r) 72,000. This predicted protein sequence contains several domains that have extensive sequence identity with s...
متن کاملTranslocation of E. coli RecQ helicase on single-stranded DNA.
A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, using ssDNA of varying lengths, the steady-state ATP hydrolysis activity of Rec...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2006
ISSN: 0021-9258
DOI: 10.1074/jbc.m513089200